-
-
22:35
»
Developmental Biology
Publication year: 2010
Source: Developmental Biology, In Press, Accepted Manuscript, Available online 3 September 2010
I.-Ching, Wang , Yufang, Zhang , Jonathan, Snyder , Mardi J., Sutherland , Michael S., Burhans , ...
Foxm1 is a member of the Forkhead Box (Fox) family of transcription factors. Foxm1 (previously called Foxm1b, HFH-11B, Trident, Win, or MPP2) is expressed in multiple cell types and plays important roles in cellular proliferation, differentiation and tumorigenesis. Genetic deletion of Foxm1 from mouse respiratory epithelium during initial stages of lung development inhibits lung maturation and causes respiratory failure after birth. However, the role of Foxm1 during postnatal lung morphogenesis remains unknown. In the present study, Foxm1 expression was detected in epithelial cells of conducting and peripheral airways and changing dynamically with lung maturation. To discern the biological role of...
-
22:35
»
Developmental Biology
Publication year: 2010
Source: Developmental Biology, In Press, Accepted Manuscript, Available online 3 September 2010
Yusuke, Sakurai , Daisuke, Kurokawa , Hiroshi, Kiyonari , Eriko, Kajikawa , Yoko, Suda , ...
Otx2 is expressed in each step and site of head development. To dissect each Otx2 function we have identified a series of Otx2 enhancers. The Otx2 expression in the anterior neuroectoderm is regulated by the AN enhancer and the subsequent expression in forebrain and midbrain later than E8.5 by FM1 and FM2 enhancers; the Otx1 expression takes place at E8.0. In telencephalon later than E9.5 Otx1 continues to be expressed in the entire pallium, while the Otx2 expression is confined to the most medial pallium. To determine the Otx functions in forebrain and midbrain development we have generated mouse mutants...
-
22:35
»
Developmental Biology
Publication year: 2010
Source: Developmental Biology, In Press, Accepted Manuscript, Available online 3 September 2010
James B., Tee , Yohan, Choi , Mita M., Shah , Ankur, Dnyanmote , Derina E., Sweeney , ...
Embryonic kidney development begins with the outgrowth of the ureteric bud (UB) from the Wolffian duct (WD) into the adjacent metanephric mesenchyme (MM). Both a GDNF-dependent and GDNF-independent pathway have been identified. In vivo and in vitro, the GDNF-dependent pathway is inhibited by BMPs, one of the factors invoked to explain the limitation of UB formation in the unbudded regions of the WD surrounding the UB. However, the exact mechanism remains unknown. Here a previously described in vitro system that models UB budding from the WD was utilized to study this process. Because PKA activation has been shown to prevent...
-
22:35
»
Developmental Biology
Publication year: 2010
Source: Developmental Biology, In Press, Accepted Manuscript, Available online 3 September 2010
Kryn, Stankunas , Gene K., Ma , Frank J., Kuhnert , Calvin J., Kuo , Ching-Pin, Chang
Heart valve malformations are one of the most common types of birth defects, illustrating the complex nature of valve development. Vascular endothelial growth factor (VEGF) signaling is one pathway implicated in valve formation, however its specific spatial and temporal roles remain poorly defined. To decipher these contributions, we use two inducible dominant negative approaches in mice to disrupt VEGF signaling at different stages of embryogenesis. At an early step in valve development, VEGF signals are required for the full transformation of endocardial cells to mesenchymal cells (EMT) at the outflow tract (OFT) but not atrioventricular canal (AVC) endocardial cushions. This...
-
22:35
»
Developmental Biology
Publication year: 2010
Source: Developmental Biology, In Press, Accepted Manuscript, Available online 3 September 2010
Huojun, Cao , Sergio, Florez , Melanie, Amen , Tuong, Huynh , Ziedonis, Skobe , ...
Tbx1-/- mice present with phenotypic effects observed in DiGeorge syndrome patients however, the molecular mechanisms of Tbx1 regulating craniofacial and tooth development are unclear. Analyses of the Tbx1 null mice reveal incisor microdontia, small cervical loops and BrdU labeling reveals a defect in epithelial cell proliferation. Furthermore, Tbx1 null mice molars are lacking normal cusp morphology. Interestingly, p21 (associated with cell cycle arrest) is up regulated in the dental epithelium of Tbx1-/- embryos. These data suggest that Tbx1 inhibits p21 expression to allow for cell proliferation in the dental epithelial cervical loop, however Tbx1 does not directly regulate p21 expression....
-
22:35
»
Developmental Biology
Publication year: 2010
Source: Developmental Biology, In Press, Accepted Manuscript, Available online 3 September 2010
Monika, Bialecka , Valerie, Wilson , Jacqueline, Deschamps
Cdx transcription factors are required for axial extension. Cdx genes are expressed in the posterior growth zone, a region that supplies new cells for axial elongation. Cdx2+/-Cdx4-/- (Cdx2/4) mutant embryos show abnormalities in axis elongation from E8.5, culminating in axial truncation at E10.5. These data raised the possibility that the long term axial progenitors of Cdx mutants are intrinsically impaired in their ability to contribute to posterior growth. We investigated whether we could identify cell-autonomous defects of the axial progenitor cells by grafting mutant cells into a wild type growth zone environment. We compared the contribution of GFP-labeled mutant and...
-
22:35
»
Developmental Biology
Publication year: 2010
Source: Developmental Biology, In Press, Accepted Manuscript, Available online 3 September 2010
Zai, Chang , Qin, Zhang , Qiuting, Feng , Jie, Xu , Teng, Teng , ...
The PI3K-PDK1-PKB/Akt (PI3K, phosphoinositide-3 kinase; PDK1, phosphoinositide-dependent protein kinase 1; PKB, protein kinase B) signaling pathway plays a critical role in a variety of biological processes including cell survival, growth and proliferation, metabolism and organogenesis. Previously, we generated Akt1-deficient mice and found high neonatal mortality with unknown causes. Here we report that histological analysis of Akt1-deficient embryos and newborns revealed heart defects and decreased cell proliferation. Echocardiographic study of Akt1-deficient mice indicated decreased heart function. Further investigation revealed that Akt1 deficiency caused substantial activation of p38MAPK in the heart. Breeding the Akt1-deficient mice to mice that were heterozygous for a...
-
22:35
»
Developmental Biology
Publication year: 2010
Source: Developmental Biology, In Press, Accepted Manuscript, Available online 3 September 2010
Margrit, Schubiger , Anne, Sustar , Gerold, Schubiger
Imaginal discs of Drosophila have the remarkable ability to regenerate. After fragmentation wound healing occurs, ectopic wg is induced and a blastema is formed. In some, but not all fragments, the blastema will replace missing structures and a few cells can become more plastic and transdetermine to structures of other discs. A series of systematic cuts through the first leg disc revealed that a cut must transect the dorsal-proximal disc area and that the fragment must also include wg-competent cells. Fragments that fail to both transdetermine and regenerate missing structures will do both when provided with exogenous Wg, demonstrating the...
Lancet (2937 unread)
Nature (5035 unread)
Science (4262 unread)
Cell
(865 unread)Genes Development (394 unread)
Developmental Dynamics (817 unread)
Mech Dev (230 unread)
EMBO J (711 unread)
PNAS (6474 unread)
JCB (674 unread)
MCB (670 unread)
Genetics (553 unread)
Science News (1507 unread)
(12 unread)
